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Appleford Railway Bridge carries the Cherwell Valley Line from Didcot to Oxford across the River Thames near the village of Appleford-on-Thames, Oxfordshire, England. It crosses the Thames on the reach between Clifton Lock and Culham Lock.
The bridge was under construction in December 1843.
Brunel's original bridge (1843) was a timber construction, replaced in the 1850s by an iron girder bridge.